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Aβ(M1-40) and Wild-Type Aβ40 Self-Assemble into Oligomers with Distinct Quaternary Structures.

Molecules. 2019; 
Bouchard JL, Davey TC, Doran TM,.
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Biochemicals … no 24612) using the manufacturer's recommended protocol WT Aβ40 monomer preparation Aβ40 was obtained commercially as a film evaporated from a solution of hexafluoro-2- propanol (HFIP) (GenScript, cat no RP1004, Piscataway, NJ, USA) … Get A Quote

摘要

Amyloid-β oligomers (AβOs) self-assemble into polymorphic species with diverse biological activities that are implicated causally to Alzheimer's disease (AD). Synaptotoxicity of AβO species is dependent on their quaternary structure, however, low-abundance and environmental sensitivity of AβOs in vivo have impeded a thorough assessment of structure-function relationships. We developed a simple biochemical assay to quantify the relative abundance and morphology of cross-linked AβOs. We compared oligomers derived from synthetic Aβ40 (wild-type (WT) Aβ40) and a recombinant source, called Aβ(M1-40). Both peptides assemble into oligomers with common sizes and morphology, however, the predominant quaternary s... More

关键词

Alzheimer’s disease; Amyloid-β; PICUP; conformation; high-molecular weight (HMW) oligomers; low-molecular weight (LMW) oligomers; morphology; oligomers; quaternary structure; recombinant Aβ