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Dendritic Cells Targeting Lactobacillus plantarum Strain NC8 with a Surface-Displayed Single-Chain Variable Fragment of CD11c Induce an Antigen-Specific Protective Cellular Immune Response.

Infect Immun. 2020; 
Liu J,, Yang G, Huang H, Shi C, Gao X, Yang W, Zhang Z, Liu Y, Xu K, Wang J, Kang Y, Jiang Y, Wang C.
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Monoclonal Antibody Services … 135 Western blotting was performed using an anti-rabbit H1N1 HA monoclonal antibody 136 (GenScript, China) and a rabbit anti-His monoclonal antibody (Abbkine, USA) as the 137 primary antibodies, followed by goat anti-rabbit IgG-HRP as the secondary antibody 138 … Get A Quote

摘要

Influenza A virus (H1N1) is an acute, highly contagious respiratory virus. The use of lactic acid bacteria (LAB) to deliver mucosal vaccines against influenza virus infection is a research hot spot. In this study, two recombinant Lactobacillus plantarum strains expressing hemagglutinin (HA) alone or coexpressing aCD11c-HA to target HA protein to dendritic cells (DCs) by fusion to an anti-CD11c single-chain antibody (aCD11c) were constructed. The activation of bone marrow dendritic cells (BMDCs) by recombinant strains and the interaction of activated BMDCs and sorted CD4+ or CD8+ T cells were evaluated through flow cytometry in vitro, and cellular supernatants were assessed by using an enzyme-linked immunosorben... More

关键词

Lactobacillus plantarum; cellular immune response; dendritic cell targeting