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Subcellular Localization of MicroRNAs by MicroRNA In Situ Hybridization (miR-ISH).

Methods Mol Biol. 2019; 
Robinson HR,, Hill MMC,, Cristino AS,.
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Molecular Biology Tools … spectra In the case of low target expression, double tagging both ends of the probe may assist detection Negative control probes were designed by using the Genscript scrambler software tool [17] for one of the target probes The … Get A Quote

摘要

MicroRNAs (miRNAs) are 22-nucleotide RNA sequences that regulate up to 60% of the mammalian transcriptome. Although canonical miRNA-induced silencing complex-mediated messenger RNA degradation occurs in the cytoplasm, miRNAs have been described in other subcellular compartments with potentially novel functions. Currently, there are limited methodologies for visualizing RNA locations within cells to elucidate mechanisms and pathways of miRNA biogenesis, transport, and function. Here, we describe a simple and rapid miRNA in situ hybridization method that can be combined with standard immunofluorescence procedures for subcellular localization of mature and precursor miRNAs.

关键词

Hybridization; MicroRNA; Microscopy; RNA; Subcellular localization