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Polycalin is involved in the toxicity and resistance to Cry1Ac toxin in Helicoverpa armigera (Hübner).

Arch Insect Biochem Physiol. 2020;

Wang B,, Wei J, Wang Y, Chen L, Liang G.

Products/Services Used	Details	Operation
Polyclonal Antibody Services	… anti-polycalin antisera 25 Production of antisera and Western blot assays Purified polycalin (3 mg) fragments were used as antigens to raise polyclonal antisera in rabbits (Genscript, Nanjing, China) Western blot analysis …	Get A Quote

摘要

Polycalin has been confirmed as a binding protein of the Cry toxins in a few Lepidoptera insects, but its function in the action mechanism of Cry1Ac and whether it is involved in resistance evolution are still unclear. In this study, Ligand blot and enzyme-linked immunosorbent assays showed that Helicoverpa armigera polycalin could specifically interact with Cry1Ac with a high affinity (Kd = 118.80 nM). Importantly, antisera blocking polycalin in H. armigera larvae decreased the toxicity of Cry1Ac by 31.84%. Furthermore, the relative gene and protein expressions were lower in Cry1Ac-resistant strain (LF60) than that in Cry1Ac-susceptible strain (LF). These findings indicated that H. armigera polycalin wa... More