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Porcine epidemic diarrhea virus ORF3 protein causes endoplasmic reticulum stress to facilitate autophagy.

Vet Microbiol. 2019; 
Zou D, Xu J, Duan X, Xu X, Li P, Cheng L, Zheng L, Li X, Zhang Y, Wang X, Wu X, Shen Y, Yao X, Wei J, Yao L, Li L, Song B, Ma J, Liu X, Wu Z, Zhang H, Cao H.
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ORF cDNA Clones/MolecularCloud … 3A) Subcellular localization of ORF3 was also predicted in silico by WoLFPSORT program (https://wwwgenscriptcom/wolf-psorthtml), and results revealed that plas: 27, mito: 3, nucl: 1, cyto: 1, indicating that the protein is likely to be associated with membranes system … Get A Quote

摘要

Porcine epidemic diarrhea virus (PEDV), the causative agent of PED, is an enveloped, positive-stranded RNA virus in the genus Alphacoronavirus, family Coronaviridae, order Nidovirales. PEDV non-structural accessory protein ORF3 is an ion channel related to viral infectivity and pathogenicity. Our previous study showed that PEDV ORF3 has expression characteristic of aggregation in cytoplasm, but its biological function remains elusive. Thus in this study, we initiated the construction of various vectors to express ORF3, and found ORF3 localized in the cytoplasm in the aggregation manner. Subsequently, confocal microscopy analysis showed that the aggregated ORF3 localized in endoplasmic reticulum (ER) to trigger ... More

关键词

Accessory protein ORF3; Apoptosis; Autophagy; Endoplasmic reticulum stress; Porcine epidemic diarrhea virus