| Products/Services Used | Details | Operation |
|---|---|---|
| DNA Sequencing | … annealing temperature varied depending on the primers used (Table 1) All PCR products were verified on a 1% agarose gel, purified using the QIAquick PCR Purification Kit (Qiagen, Germantown, Maryland), and sent to GenScript (https://wwwgenscriptcom) for sequencing to … | Get A Quote |
Coral disease is a major threat to reef ecosystems and therefore, understanding the cellular pathways underlying disease progression and resistance is critical to mitigating future outbreaks. This study focused on tyrosinase-like proteins in cnidarians, which contribute to melanin synthesis, an invertebrate innate immune defense. Specifically, characterization and phylogenetic analysis of cnidarian tyrosinases were performed, and their role in symbiosis and a "mystery disease" in the anemone Exaiptasia pallida was investigated using qPCR. The results reveal a diversity of tyrosinase-like proteins in cnidarians that separate into two major clades on a phylogenetic tree, suggesting functional divergence. Two E. p... More
