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Isolation and Expression Analysis of Three Types of α-Carbonic Anhydrases from the Antarctic Alga Chlamydomonas sp. ICE-L under Different Light Stress Treatments.

Mol Biotechnol. 2019; 
Shi C,, An M,,, Miao J,,, He Y,, Zheng Z,,, Qu C, Wang X, Lin H, Liu J.
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Molecular Biology Tools … Then, protein subcellular localisation was analysed using prediction tools (https://wwwgenscript com/wolf-psorthtml), and the theoretical isoelectric point (pI) and molecular weight (Mw) of each target protein were calculated with the Compute pI/Mw tool of ExPASy ProtParam … Get A Quote

摘要

Carbonic anhydrases (CAs) are a class of zinc-containing metalloenzymes that can reversibly catalyse the hydration reaction of carbon dioxide. Antarctic algae are the most critical component of the Antarctic ecosystem; algae can enter the carbon cycle food chain by fixing carbon dioxide from the air. In this study, the complete open reading frames (ORFs) of CA1 (GenBank ID KY826431), CA2 (GenBank ID KY826432), and CA3 (GenBank ID KY826433), encoding CAs in the Antarctic ice microalga Chlamydomonas. sp. ICE-L, were successfully cloned using reverse transcription-polymerase chain reaction (RT-PCR). In addition, the expression patterns of CAs under blue light, under UV light, and in the dark were determined by qua... More

关键词

Bioinformatic analysis; Carbonic anhydrase; Chlamydomonas sp. ICE-L; qRT-PCR