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Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens

Int J Mol Sci. 2020-04; 
Yip CC, Ho CC, Chan JF, To KK, Chan HS, Wong SC, Leung KH, Fung AY, Ng AC4 Zou Z, Tam AR, Chung TW, Chan KH, Hung IF, Cheng VC, Tsang OT, Tsui SKW, Yuen KY
Products/Services Used Details Operation
Molecular Biology Tools The four reported targets were subjected to analysis using the GenScript real-time PCR (TaqMan) Primer and Probes Design Tool (https://www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool) with default settings. Only one of the targets fulfilled the primer and probe design criteria. Get A Quote

摘要

The pandemic novel coronavirus infection, Coronavirus Disease 2019 (COVID-19), has affected at least 190 countries or territories, with 465,915 confirmed cases and 21,031 deaths. In a containment-based strategy, rapid, sensitive and specific testing is important in epidemiological control and clinical management. Using 96 SARS-CoV-2 and 104 non-SARS-CoV-2 coronavirus genomes and our in-house program, GolayMetaMiner, four specific regions longer than 50 nucleotides in the SARS-CoV-2 genome were identified. Primers were designed to target the longest and previously untargeted nsp2 region and optimized as a probe-free real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. The new COVID... More

关键词

COVID-19; COVID-19-nsp2 assay; GolayMetaMiner; SARS-CoV-2; clinical evaluation; genome subtraction; nsp2; real-time RT-PCR; sensitivity; specificity