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Co-delivery of NS1 and BMP2 mRNAs to murine pluripotent stem cells leads to enhanced BMP-2 expression and osteogenic differentiation

Acta Biomater. 2020-04; 
Wang P, Logeart-Avramoglou D, Petite H, Goncalves C, Midoux P, Perche F, Pichon C
Products/Services Used Details Operation
ORF cDNA Clones/MolecularCloud Mouse BMP-2 ORF was PCR amplified from pCMV3-mBMP2-GFPSpark (Sino Biologicals) with 5′-TATGGATCCACTTAAGATGGTGGCCGGGACCCGCTGT-3′ as forward primer and 5′-TATTGCGGCCGCTTAACGACACCCGCAGCCCTC-3′ as reverse primer (Eurogentec). NS1 (A/Texas/36/1991) ORF was PCR amplified from pUC57-NS1 (Genscript) with 5′-tgtacggatcctcctatggattccaacactgtgtc-3′ and 5′-atttgcggccgctcaaacttctgacct-3′ as the forward primer and reverse primer (Eurogentec), respectively. Get A Quote

摘要

Application of messenger RNA (mRNA) for bone regeneration is a promising alternative to DNA, recombinant proteins and peptides. However, exogenous in vitro transcribed mRNA (IVT mRNA) triggers innate immune response resulting in mRNA degradation and translation inhibition. Inspired by the ability of viral immune evasion proteins to inhibit host cell responses against viral RNA, we applied non-structural protein-1 (NS1) from Influenza A virus (A/Texas/36/1991) as an IVT mRNA enhancer. We evidenced a dose-dependent blocking of RNA sensors by NS1 expression. The co-delivery of NS1 mRNA with mRNA of reporter genes significantly increased the translation efficiency. Interestingly, unlike the use of nucleosides... More

关键词

Bone morphogenetic protein 2; Non-structural protein 1; Osteogenesis; RNA sensors; mRNA delivery