| Products/Services Used | Details | Operation |
|---|---|---|
| ORF cDNA Clones/MolecularCloud | … by 15 min at 70°C. For PCR, 2 μl of the cDNA was used in a reaction mixture containing 500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25°C), 15 mM MgCl 2 , 1% Triton X-100, 0.2 mM each dNTP, 0.2 μΜ of each primer, 1 U of Taq DNA Polymerase (GenScript, Piscataway, USA) and … | Get A Quote |
RNA silencing is a major antiviral mechanism in plants, which is counteracted by virus-encoded proteins with silencing suppression activity. ORFs encoding putative silencing suppressor proteins that share no structural or sequence homology have been identified in the genomes of four criniviruses. In this study, we investigated the RNA silencing suppression activity of several proteins encoded by the RNA1 (RdRp, p22) and RNA2 (CP, CPm and p26) of cucurbit chlorotic yellows virus (CCYV) using co-agroinfiltration assays on Nicotiana benthamiana plants. Our results indicate that p22 is a suppressor of local RNA silencing that does not interfere with cell-to-cell movement of the RNA silencing signal or with systemic... More
