Products/Services Used | Details | Operation |
---|---|---|
Molecular Biology Tools> | … sequencing data. The DNA fragment comprising exlB (PSPA7-4641), exlA (PSPA7_4642), intergenic region and the exlB RBS was synthesized by GenScript (www.genscript.com) and sub-cloned into apBad miniCTX-1 integrative vector pSW196 (Baynham et al., 2006) … | Get A Quote |
Virulence of Pseudomonas aeruginosa is typically attributed to its type III secretion system (T3SS). A taxonomic outlier, the P. aeruginosa PA7 strain, lacks a T3SS locus, and no virulence phenotype is attributed to PA7. We characterized a PA7-related, T3SS-negative P. aeruginosa strain, CLJ1, isolated from a patient with fatal hemorrhagic pneumonia. CLJ1 is highly virulent in mice, leading to lung hemorrhage and septicemia. CLJ1-infected primary endothelial cells display characteristics of membrane damage and permeabilization. Proteomic analysis of CLJ1 culture supernatants identified a hemolysin/hemagglutinin family pore-forming toxin, Exolysin (ExlA), that is exported via ExlB, representing a putative tw... More