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LncRNA TTN-AS1 acts as sponge for miR-15b-5p to regulate FBXW7 expression in ovarian cancer

Biofactors. 2020; 
Miao S, Wang J, Xuan L, Liu X.
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ORF cDNA Clones/MolecularCloud … 22 Cell transfection pcDNA31 contains TTN‐AS1 (pcTTN‐AS1) was obtained from GenScript (Nanjing, Jiangsu, China) Small interfering RNA against F‐box and WD repeat domain containing 7 (FBXW7, si‐FBXW7, 5′‐CCAUGCAAAGUCUCAGAAU … Get A Quote

摘要

Emerging evidence showed that long noncoding RNA (lncRNA) plays crucial roles in regulating various cancer biological behaviors. Titin-antisense RNA1 (TTN-AS1) has been reported to have crucial roles in cancers but its role in ovarian cancer remains unknown. The levels of TTN-AS1, microNRA-15b-5p (miR-15b-5p), and F-box and WD repeat domain containing 7 (FBXW7) in ovarian cancer cells were measured by quantitative reverse-transcription PCR. Targets for TTN-AS1 and miR-15b-5p were predicted by bioinformatic tools, and validated by luciferase activity reporter assay. Cell proliferation, colony formation, and cell apoptosis were analyzed with cell counting kit-8 assay, colony formation assay, and flow cytometry. C... More

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