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RNA-Binding Protein Rnc1 Regulates Cell Length at Division and Acute Stress Response in Fission Yeast through Negative Feedback Modulation of the Stress-Activated Mitogen-Activated Protein Kinase Pathway

MBio. 2020; 
Prieto-Ruiz F, Vicente-Soler J, Franco A, Gómez-Gil E, Sánchez-Marinas M, Vázquez-Marín B, Aligué R, Madrid M, Moreno S, Soto T, Cansado J.
Products/Services Used Details Operation
ORF cDNA Clones/MolecularCloud To obtain plasmids pTA-Rnc1(K110D, A111D, R196D, N197D, R338D, G339D):HA [synonymous with Rnc1(mKH):HA; Rnc1 mutated at the 3 KH mRNA binding domains] and pTA-Rnc1(T45A, T50A, T171A, T177A, S278A, S286A):HA [synonymous with Rnc1(S/T6A):HA; Rnc1 mutant lacking MAPK phosphosites], plasmid pTA- Rnc1:HA was digested with SacI and PacI, and the released rnc1⫹ ORF fragment was replaced with synthesized DNA fragments including the indicated mutations (GenParts; GenScript) and digested with SacI and PacI. Get A Quote

摘要

RNA-binding proteins (RBPs) play a major role during control of mRNA localization, stability, and translation and are central to most cellular processes. In the fission yeast Schizosaccharomyces pombe, the multiple K homology (KH) domain RBP Rnc1 downregulates the activity of the cell integrity pathway (CIP) via stabilization of pmp1 + mRNA, which encodes the Pmp1 phosphatase that inactivates Pmk1, the mitogen-activated protein kinase (MAPK) component of this signaling cascade. However, Rnc1 likely regulates the half-life/stability of additional mRNAs. We show that Rnc1 downregulates the activity of Sty1, the MAPK of the stress-activated MAPK pathway (SAPK), during control of cell length at division and recover... More

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