| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Partial 16S rRNA gene fragments were amplified by polymerase chain reaction (PCR) using the bacterial primers 27F: 5’-AGAGTTTGATCCTGGCTCAG-3’ and 1541R: 5’-AAGGAGGTGATCCAGCCGCA-3’. Amplification was carried out in a DNA Engine thermal cycler (BIORAD, USA), using a 50-µL reaction mixture containing 4 µL Taq DNA polymerase (2.5 U µL-1, Genscript, Nanjing), 5 µL 10× buffer (Transgene, Beijing), 1 µL 20 mM deoxynucleoside triphosphate (Transgene), 37µL of sterile distilled water, 1 µL of each primer (50 µM), and 1 µL of template. The PCR thermocycling conditions were as follows: initial denaturation at 94°C for 4 min; 30 cycles at 94°C for 1 min, 56°C for 1 min, 72°C for 2 min; and a final elongation at 72°C for 10 min. PCR reactions were purified and sequenced by Genscript Biotech (Nanjing) Co., Ltd, China. | Get A Quote |
Actinomycetes are important producers of novel bioactive compounds. New sources need to be explored for isolating previously unknown bioactive compound-producing actinomycetes. Here we evaluated the potential of bark as a natural source of novel bioactive actinomycete species. Bark samples were collected from nine tree species at different elevations (1600-3400 m a.s.l.) on Qin Mountain, Shaanxi Province, China. Actinomycetes were cultivated, enumerated and isolated using serial dilution and spread-plate techniques. The antimicrobial activity of actinomycete isolates was analyzed using an agar block method against 15 typical bacterial and fungal species and plant pathogens. The dominant isolates were identified... More
