| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Peptide samples were custom-synthesized from GenScript (Piscataway, NJ, USA) at a purity between 90 and 99%. For NMR, the lyophilized powders were dissolved in a mixture of H2O/D2O (9:1) to a concentration of 1 to 2 mM. Because of a better solubility, the biotinylated AcpP-derived peptide P1 was dissolved in a 10 mM acetate buffer solution at pH 4.9. | Get A Quote |
The influence of biotin and fluorescein tags attached to the N-terminus of peptides on their structural propensities is assessed by NMR methods. While the small peptides investigated are highly mobile with no uniquely preferred conformation, the introduction of the tags, in particular hydrophobic ones, clearly shows an influence on NMR parameters such as chemical shifts and relaxation properties, which are not restricted to the nearby residues, but also affect distant parts. Thus, long-range effects on structural propensities become evident and are cause for concern with respect to the interpretation of weak interaction tests, which rely upon the assumption that tags do not exert influence on intermolecular int... More
