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Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency

Genome Biol. 2019; 
Zhao T, , Huan Q, Sun J, Liu C, Hou X, Yu X, Silverman IM, Zhang Y, Gregory BD, Liu CM, Qian W, , Cao X0,
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Custom DNA/RNA Oligos We followed the protocol of EMSA/Gel-Shift Kit (Beyotime, GS002) with modifications. The oligo-RNAs were synthesized chemically by Genscript (Nanjing, China). Get A Quote

摘要

Polyadenylation plays a key role in producing mature mRNAs in eukaryotes. It is widely believed that the poly(A)-binding proteins (PABs) uniformly bind to poly(A)-tailed mRNAs, regulating their stability and translational efficiency.,We observe that the homozygous triple mutant of broadly expressed Arabidopsis thaliana PABs, AtPAB2, AtPAB4, and AtPAB8, is embryonic lethal. To understand the molecular basis, we characterize the RNA-binding landscape of these PABs. The AtPAB-binding efficiency varies over one order of magnitude among genes. To identify the sequences accounting for the variation, we perform poly(A)-seq that directly sequences the full-length poly(A) tails. More than 10% of poly(A) tails contain at... More

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