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Anti-inflammatory action of cysteine derivative S-1-propenylcysteine by inducing MyD88 degradation

Sci Rep. 2018; 
Suzuki JI, Kodera Y, Miki S, Ushijima M, Takashima M, Matsutomo T, Morihara N
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ORF cDNA Clones/MolecularCloud Te supernatants were collected. Cell lysates were treated with S1PC (0.3, 1 and 3 mM) or SAC (3 mM) for 10-60 min at 37 °C. 293FT cells were transfected with pcDNA3.1+/c-(k) dyk containing MyD88 (Genscript, NJ, USA). Get A Quote

摘要

The degradation of target proteins by small molecules utilizing the cellular proteolytic system is featured as a treatment strategy of several diseases. We found that S-1-propenylcysteine (S1PC) among several cysteine derivatives in aged garlic extract inhibited TLR-mediated IL-6 production by inducing the degradation of adaptor protein MyD88. We showed that S1PC directly denatured MyD88 and induced the formation of protein aggregates. Consequently, MyD88 was degraded by aggresome-autophagy pathway. On the other hand, S-allylcysteine, a structural analog of S1PC, failed to induce the degradation of MyD88 because of its inability to denature MyD88 although it also activated autophagy. Our findings suggest that S... More

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