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Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei

Aquaculture Research. 2020; 
Han Fang, | Xinwei Liu | Xuqing Shao | Fang Xin, | Baojie Wang, | Mengqiang Wang, | Keyong Jiang, | Mei Liu, | Lei Wang,
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摘要

Raptor, a member of the target of rapamycin complex 1 (TORC1), participates in the formation of complex proteins related to the mechanistic target of rapamycin (mTOR) signalling. In this study, a 5,020 bp cDNA of Raptor with an open reading frame (ORF) of 3,804 bp encoding for 1,267 amino acids was cloned from Litopenaeus vannamei. The protein contains three conserved domains: Raptor N, HEAT and WD40 domains. The expression of Raptor gene was detected by qRT‐PCR in different tissues of L. vannamei, including hepatopancreas, intestinal, stomach, eyestalk, gill and muscle. The mRNA expression profiles of Raptor in muscle were also analysed under suppression or stimulation of mTOR signalling pathway. ... More

关键词

gene expression, Litopenaeus vannamei, molecular characterization, Raptor, RNA interference