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Synthesis, characterisation, and application of chamomile gold nanoparticles in molecular diagnostics: a new component for PCR kits

Biointerface Research in Applied Chemistry. 2020-04; 
Mohammad Jafar Maleki, Mohammad Pourhassan-Moghaddam, Abbas Karimi, Seyed Abolghasem Mohammadi
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Molecular Biology Tools Primers of Human Beta-actin gene and bacterial MecA gene were designed using the online sofeware (https://www.genscript.com/tools/pcr-primers-designer). Get A Quote

摘要

Various strategies have been suggested for successful amplification of the hard-to-amplify nucleic acids such as the inclusion of various chemical or biological materials in the in vitro nucleic acid amplification reactions, particularly polymerase chain reaction (PCR), and adjustment of the cycling programs. Although much efforts have been made for improvements in the enhancement of polymerase chain reactions of high GC content nucleic acids, still significant challenges remain. In this study, the effects of citrate-coated AuNP and chamomile extract-coated AuNP (p-AuNP) on amplification of three genes with significant different GC percentage were evaluated. Owing to the enormous potential of gold nanoparticles... More

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