Enhancement of Tumor-Specific T Cell–Mediated Immunity in Dendritic Cell–Based Vaccines by Mycobacterium tuberculosis Heat Shock Protein X

Recombinant mouse GM-CSF, IL-4, CCL19, and the FITC–Annexin V/propidium iodine kit were purchased from R&D Systems (Minneapolis, MN).Dextran-FITC (40,000 Da) was purchased from Sigma-Aldrich (St. Louis,MO). LPS (from Escherichia coli O111:B4) was purchased from Invivogen(San Diego, CA). H-2Kb–restricted OVA peptide (OVA257–264), H-2Db–restricted OVA peptide (OVA323–339), and HPV-16 E7 (aa 49–57) peptide(RAHYNIVTF) were synthesized by Peptron (Daejeon, Korea). The followingFITC- or PE-conjugated mAbs were purchased from BD Biosciences (SanJose, CA): CD8 (SK17), CD11c (HL3), CD62L (MEL14), CD80 (16-10A1),CD83 (Michel-17), CD86 (GL1), Iab b-chain (AF-120.1), H-2Kb (AF6-88.5),CCR7 (CD197), IL-10 (JESS-16E3), and IL-12p40/p70 (C15.6). Alexa 568–conjugated anti-mouse IgG Abs were purchased from Invitrogen (Eugene,OR). FITC-conjugated mouse IgG Abs and cytokine ELISA kits for murineIL-1b, IL-2, IL-6, IL-10, IL-12p70, TNF-a, IFN-b, and IFN-g were purchased from eBiosciences (San Diego, CA).