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Optimizing T4 DNA polymerase conditions enhances the efficiency of one-step sequence-and ligation-independent cloning

BioTechniques. 2018-03; 
Mohammad Nazrul Islam, Kyeong Won Lee, Hyung-Soon Yim, Seong Hyuk Lee, Hae Chang Jung, Jung-Hyun Lee, and Jae-Yeon Jeong
Products/Services Used Details Operation
ORF cDNA Clones/MolecularCloud … Many commercial kits, including In-Fusion (Clontech, Mountain View, CA) and CloneEZ cloning (GenScript, Piscataway, NJ), have been developed based on the same principles as SLIC (1). Although … Cited By: Middle East respiratory syndrome coronavirus-encoded ORF8b … Get A Quote

摘要

关键词

seamless cloning; SLIC; T4 DNA polymerase
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