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AN OVERVIEW ON “GenSmart”–A SMART DNA CONSTRUCT DESIGN TOOL

bviorxiv. 2020; 
Siddharth R V, Shankar M & Karthikeyan A
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摘要

Direct synthesis of genes is rapidly becoming the most efficient way to make functional genetic constructs and enables applications such as RNAi resistant genes and protein engineering. In this paper, a newly developed DNA construct and design tool “GenSmart” has been trialed using Bacillus subtilis aprE mRNA for protease, partial cds, strain : KIPI16 and Bacillus subtilis strain MA139 beta-1,3- 1,4-glucanase mRNA, partial cds , for insertion process.

关键词

DNA/RNA, mRNA, de novo , plasmid map, synthetic biology, gene synthesis, nucleotide sequence.