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CCK1, a PMK1-type MAP kinase is required for hyphal growth, pigmentation, conidiation, enzyme activity, osmotic stress response, and pathogenicity in Corynespora …

European Journal of Plant Pathology. 2017-03; 
Xiaomei Liu, Shenwen Cao, He Zhang, Yunxie Wei & Jinji Pu
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GenParts™ DNA Fragments … The resulting PCR product was cloned into pFB in the antisense orientation via the SalI site with a CloneEZ® PCR Cloning Kit (GenScript, Hong Kong, China) This yielding a recombinant vector pΔcck1, in which the hygB gene was flanked by cck1 fragments (Fig 1a) … Get A Quote

摘要

Corynespora cassiicola is a fungal pathogen causing Corynespora Leaf Fall disease (CLF) in rubber trees (Hevea brasiliensis). CLF has great economic impact in rubber-producing countries. However, the molecular mechanisms involved in the pathogenicity of C. cassiicola remain poorly understood. Mitogen-activated protein kinase (MAPK) is one of the major signaling pathways responsible for regulation of fungal development and virulence in many plant fungal pathogens. The C. cassiicola cck1 gene encodes a MAPK that is homologous to the rice pathogen Magnaporthe grisea MAPK Pmk1 protein. In this study, targeted cck1 gene disruption and complementation experiments were carried out in C. cassiicola. Compared to the wil... More

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