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PlWRKY70: a Paeonia lactiflora transcription factor that sensitively responds to low-temperature, salt and waterlogging stresses

Canadian Journal of Plant Science. 2019-09; 
Caiyun Han,a* Junjie Li,a* Yan Ma,a Jing Guo,a Xianfeng Guo,a Jinguang Xub
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摘要

The WRKY family is a specific super gene family in plants that plays a significant regulatory role in abiotic stress in plants. In this paper, the PlWRKY70 gene was cloned by RT-PCR from Paeonia lactiflora ‘Da Fugui’ buds (GenBank accession no. KU891819). The open reading frame of the gene was 936 bp in length, encoding 311 amino acids. The PlWRKY70 gene contained a WRKY domain in its coding region that belonged to the group III WRKY family and was evolutionarily the closest to Paeonia suffruticosa. PlWRKY70 was widely expressed and found at an extremely high level in buds. Moreover, the PlWRKY70 protein was mostly detected in the nucleus. The expression of PlWRKY70 was remarkably influenced by different ab... More

关键词

Paeonia lactiflora, PlWRKY70, cloning, subcellular localization, expression analysis, abiotic stress