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Multi-scale ecological filters shape the crayfish microbiome

Symbiosis. 2016-12; 
James Skelton, Kevin M. Geyer, Jay T. Lennon, Robert P. Creed & Bryan L. Brown
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Biochemicals … Amplifications were digested with HaeIII (New England BioLabs, Ipswich, MA, USA) in triplicate (20 μL reaction volume) for 3 h at 37 °C following manufacturer's suggested protocols Digestion replicates were then pooled and cleaned using GenScript extraction kits … Get A Quote

摘要

A general and practical understanding of the processes that drive microbiome assembly and structure are paramount to understanding organismal biology, health, and evolution. In this study of stream-dwelling crayfish, we conceptualized colonization of microbial symbionts as a series of ecological filters that operate at the environment, host, and host microsite levels, and identified key ecological processes at each level. A survey of Cambarus sciotensis in western Virginia, USA, showed that the local environment and host microsites interact to create complex patterns of microbial diversity and composition. An in situ experiment confirmed a prevailing effect of host microsite on microbial composition, and also s... More

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