Products/Services Used | Details | Operation |
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GenParts™ DNA Fragments> | Following amplification, the DNA fragments were purified using the QIAEX II Gel Extraction Kit (Qiagen) or ExoSAP-IT (Affymetrix) and sent to Genewiz (South Plainfield, NJ, USA) or GenScript (Piscataway, NJ, USA) for sequencing. The raw sequences were preliminary optimized using the “Online Analysis Tools” package (http://molbiol-tools. ca). Multiple DNA sequence alignments were generated by ClustalW software [Thompson et al., 1997], and the alignment was subsequently corrected manually in MEGA 5 [Tamura et al., 2011]. | Get A Quote |
Near-term and future increases in global population overburden our energy resources as we seek solutions to unstable foreign petroleum pricing and its associated problems with climate change and ecological biosphere integrity. Renewable and sustainable biofuels have the potential to replace existing transportation fuels and dampen their related problems. Small but versatile, duckweed is an aquatic plant that can be used in a variety of applications such as biofuels, animal feed, and wastewater remediation. This dissertation addresses the need for quick and reliable typing of duckweed species and lower taxonomic levels. First, a sequence database for two plastidic barcodes, atpF-atpH and psbK-psbI was created re... More