| Products/Services Used | Details | Operation |
|---|---|---|
| DNA Sequencing | … protocol PCR to amplify the ITS2 and COI genes was then carried out as described previously (Chen et al 2017) PCR products were sent to GenScript (Nanjing, China) for purification and sequencing Amplicon sequences … | Get A Quote |
The oomycete pathogen causing root-rot and damping-off diseases in soybean (Glycine max) fields in the Huang-Huai region of China was identified as Pythium spinosum. To detect P. spinosum for disease diagnosis and control, we developed a loop-mediated isothermal amplification (LAMP) reaction with a primer set designed from the rDNA internal transcribed spacer 2 (ITS2) sequence of P. spinosum. The LAMP assay can efficiently amplify the target gene within 60 min at 63 °C. In specificity tests using 31 Pythium spp., 12 Phytophthora spp., 6 Phytopythium spp., and 9 other fungi strains, no cross-reactions were observed in the LAMP assay. The detection limit was 100 pg·μL−1 of genomic DNA per reaction. In cases ... More
