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Functional Annotation of Hypothetical Proteins Derived from Suppressive Subtraction Hybridization (SSH) Analysis Shows NPR1 (Non-Pathogenesis Related)-Like …

Agronomy. 2019-01; 
Murugesan Chandrasekaran 1, Chandrasekar Raman 2, Kandasamy Karthikeyan 3 and Manivannan Paramasivan
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Molecular Biology Tools … for analyzing permissive sites in the profile 243 Subcellular Localization PSORT (https://wwwgenscriptcom/psorthtml) tool was used for predicting subcellular localization of proteins Further, CELLO (http://cellolifenctuedu … Get A Quote

摘要

Fusarium wilt is considered the most devastating banana disease incited by Fusarium oxysporum f. sp. cubense (FOC). The present study addresses suppressive subtraction hybridization (SSH) analysis for differential gene expression in banana plant, mediated through FOC and its interaction with biocontrol agent Trichoderma asperellum (prr2). SSH analysis yielded a total of 300 clones. The resultant clones were sequenced and processed to obtain 22 contigs and 87 singleton sequences. BLAST2GO (Basic Local Alignment Search Tool 2 Gene Ontology) analysis was performed to assign known protein function. Initial functional annotation showed that contig 21 possesses p38-like endoribonuclease activity and duality in subcel... More

关键词

Fusarium oxysporum f. sp. cubense; functional annotation; Musa defense pathway; suppressive subtraction hybridization; Trichoderma asperellum