| Products/Services Used | Details | Operation |
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| Catalog Antibody | After the ascites were purified, the titer of each antibody was determined by ELISA. In brief, 100 µL of 108 CFU mL-1K. pneumoniae (inactivated) was added to each well and incubated at 37°C for 2 hours. Then, all wells were washed three times with phosphate-buffered saline (PBS) containing 0.05% tween-20, blocked with 200 µL of blocking buffer (5% skimmed milk in PBS-T) for 1 hour at 37°C, and washed as described earlier. Next, 100 µL of the three antibodies with different dilutions (from 1:200 to 1: 409600) was added to each well and maintained for 2 hour at 37°C. Then, each well was washed as described earlier and incubated with the goat anti-mouse IgG (H + L) horseradish peroxidase (HRP) (GenScript, USA) diluted for the final concentration of 0.25 µg mL-1. After washing, the soluble TMB substrate solution (TIANGEN, China) was added to each well, followed by incubation for 15 minutes at 37°C. Finally, the reaction was terminated by the stop buffer (2 M H2SO4), and the optical density of each well was measured at 450 nm. | Get A Quote |
| Catalog Antibody | Get A Quote |
Background: Klebsiella pneumoniae is an important human pathogen that causes severe diseases including urinary tract infection, pneumonia, and bacteremia. However, a rapid and sensitive detection method remains to be developed. Objectives: This study aimed to develop a rapid, real-time, and visual detection method for K. pneumoniae. This is a primary screening method to improve the diagnosis of K. pneumoniae infection and save much precious time for clinical practice. Methods: Klebsiella pneumoniae was used as an antigen to produce a monoclonal antibodies (mAb). The mAb 1E6 recognizing outer membrane protein C on the surface of K. pneumoniae was screened by an indirect enzyme-linked immunosorbent assay (E... More
