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Expression Profiles of a Tung Tree Phosphate Transporter cDNA and Structural Characteristics of the Encoded Protein

Cytology and Genetics volume. 2019-03; 
Junqin Zhou, Jun Yuan, Hongxu Long & Xiaofeng Tan
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Molecular Biology Tools … MEGA4 Furthermore, the ExPASy online tool was used to predict protein properties The protein subcellular localization was predicted using the WoLF PSORT program (https://wwwgenscriptcom/wolf-psorthtml) Protein topology … Get A Quote

摘要

Plant phosphate transporters mediate phosphate acquisition, translocation, and recycling. A Vernicia fordii cDNA encoding a phosphate transporter was cloned using a reverse transcription polymerase chain reaction (PCR) and rapid amplification of cDNA ends. The resulting cDNA named Vernicia fordii Phosphate Transporter-1;1 (VfPht1;1) encodes a predicted protein consisting of 533 amino acids. This protein, which was predicted to contain 12 transmembrane domains, is most similar (89.9%) to a Ricinus communis predicted protein. The quantitative real-time PCR data revealed that VfPht1;1 is most highly expressed in mature leaf petioles. It is also highly expressed in seeds collected in June, when developing fruits re... More

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