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Bioinformatics Analysis of the Ζ-Carotene Desaturase Gene in Potato (Solanum Tuberosum)

Advances in Biological Sciences Research. 2018; 
Jie Ma , , Ping Zhou , , Chunyan Chen , Min Jiang, Meiling Wu, Rui Wu , Chaohai Wang and Bo Sun , a, *
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Molecular Biology Tools The amino acid sequence, protein molecular weight, isoelectric point, stability index, and hydrophobicity of the StZDS gene were analyzed and predicted by ExPASy (http://web.expasy.org) and NCBI (https://www.ncbi.nlm.nih.gov/). Subcellular localization was predicted by WoLF PSORT (http://www.genscript.com/wolf-psort.html). The conserved domain were predicted by NCBI (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi). Phylogenetic tree analysis of the ZDS proteins was executed in MEGA 6.0 using the neighbor-joining (NJ) method. Get A Quote

摘要

Ζ-carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Solanum tuberosum ZDS (StZDS) gene sequences were obtained from Spud DB database, and preformed for bioinformatics analysis. The StZDS gene mapped to chromosomes 1, and contains an open reading frame of 1,767 bp that encodes a 588-amino acid protein with a calculated molecular mass of 64.70 kD and an isoelectric point (pI) of 8.6. Subcellular localization predicted the StZDS gene was in the chloroplast. The conserved domain of the StZDS protein is PLN02487. The ZDS protein is most closely related to Solanum lycopersicum. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in... More

关键词

Solanum lycopersicum; Ζ-carotene desaturase; Subcellular localization predicted.