| Products/Services Used | Details | Operation |
|---|---|---|
| DNA Sequencing | … CA, USA) according to the manufacturer's instructions. Plasmids of three independent positive clones of each sample were sequenced by GenScript (Nanjing) Co., Ltd., China. The sequences were analysed using the DNASTAR's … | Get A Quote |
Young apple trees exhibiting symptoms of little leaf, margin involute and yellows were observed in an open‐air nursery in Yangling, Shaanxi, China. Transmission electron microscopy showed typical phytoplasma bodies in the sieve tube elements of symptomatic leaf samples. In two nested polymerase chain reaction (PCR ) assays, products of expected size of 1.1 and 1.2 kb were separately generated from the total DNA s of symptomatic samples. A restriction fragment length polymorphism (RFLP ) analysis of the purified 1.2 kb PCR products indicated that the disease associated with apple trees was ‘Ca . Phytoplasma ziziphi’. To our knowledge, this is the first report of ‘Ca . Phytoplasma ziziphi’ infecting app... More
