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Towards a scaled-up T cell-mediated cytotoxicity assay in 3D cell culture using microscopy

biorxiv. 2019; 
Elinor Gottschalk, Eric Czech, Bulent Arman Aksoy, Pinar Aksoy, Jeff Hammerbacher
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Biochemicals On the day that cells were thawed for experiments, hgp100 (GenScript, Piscataway, NJ) was added to the cultures to activate the T cells at a final concentration of 1 μM. Get A Quote

摘要

Three-dimensional (3D) cell culture systems with tumor spheroids are being adopted for research on the antitumor activity of drug treatments and cytotoxic T cells. Analysis of the cytotoxic effect on 3D tumor cultures within a 3D scaffold, such as collagen, is challenging. Image-based approaches often use confocal microscopy, which greatly limits the sample size of tumor spheroids that can be assayed. We explored a system where tumor spheroids growing in a collagen gel within a microfluidics chip can be treated with drugs or co-cultured with T cells. We attempted to adapt the system to measure the death of cells in the tumor spheroids directly in the microfluidics chip via automated widefield fluorescence micro... More

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