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Biochemicals> | … Page 4 polymerase (GenScript, Piscataway, NJ) PCR was set for initial denaturation at 958C for 5 min, followed by 35 cycles of 20 sec at 958C, 45 sec at 558C, and 45 sec at 728C The reactions were finished with a final extension at 728C for 7 min … | Get A Quote |
Tar balls have been persisting along the Gulf coast. Previous research has shown that tar balls from the Gulf coast may contain the human pathogen Vibrio vulnificus. These oil residuals are also commonly found beneath sea shells (hereby called “tar shells”). No research, however, has explored the bacterial communities in tar shells. In this study, tar shell, tar ball, and sand samples on the Alabama Gulf coast at intertidal and supratidal zones were collected and their bacterial communities were assessed using PCR-DGGE and DNA sequencing. Our data suggest that Desulfovibrio and Actinobacteria spp. are dominant in tar shells at intertidal and supratidal zones, but not in tar balls at supratidal zone, while P... More