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DNA Sequencing> | … Escherichia coli was incubated overnight on Luria Bertani agar containing Kanamycin (final concentration: 50 μg/ml) at 37℃. All plasmids were validated via sequencing by GenScript, Inc.. 2.5 Agrobacterium tumefaciens infiltration assays … | Get A Quote |
The pine wood nematode (PWN), Bursaphelenchus xylophilus, causes huge economic losses in pine forests. The plant‐parasitic nematodes have a complex life cycle that includes the secretion of effector proteins through a stylet into the host cell to promote parasitism. In this study, SignalP 4.1 and TMHMM 2.0 were used in preliminary screens for candidate effectors and were expressed in Nicotiana benthamiana through the PVX virus expression vector. The yeast signal sequence trap system was used to further study the function of the signal peptide of an effector, BxSapB2. In situ hybridization was conducted to investigate the localization of BxSapB2 , followed by RNA interference technology (RNAi) to assess the fu... More