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An evaluation of COVID-19 serological assays informs future diagnostics and exposure assessment

Nat Commun. 2020-07; 
Corine H GeurtsvanKessel, Nisreen M A Okba, Zsofia Igloi, Susanne Bogers, Carmen W E Embregts, Brigitta M Laksono, Lonneke Leijten, Casper Rokx, Bart Rijnders, Janette Rahamat-Langendoen, Johannes P C van den Akker, Jeroen J A van Kampen, Annemiek A van der Eijk, Rob S van Binnendijk, Bart Haagmans, Marion Koopmans
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Serology SARS-Cov-2 detection kits … An alternative of the PRNT50 can be the use of a surrogate virus neutralization test for SARS CoV-2 (as recently produced by Genscript, USA) which allows direct quantification. Extensive studies on the performance have however not yet been published. Fig … Get A Quote

摘要

The world is entering a new era of the COVID-19 pandemic in which there is an increasing call for reliable antibody testing. To support decision making on the deployment of serology for either population screening or diagnostics, we present a detailed comparison of serological COVID-19 assays. We show that among the selected assays there is a wide diversity in assay performance in different scenarios and when correlated to virus neutralizing antibodies. The Wantai ELISA detecting total immunoglobulins against the receptor binding domain of SARS CoV-2, has the best overall characteristics to detect functional antibodies in different stages and severity of disease, including the potential to set a cut-off indicat... More

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