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Determining the immunological characteristics of a novel human monoclonal antibody developed against staphylococcal enterotoxin B

Hum Vaccin Immunother. 2020-04-01; 
Yuanyuan Liu, Zhen Song, Shuang Ge, Jinyong Zhang, Limin Xu, Feng Yang, Dongshui Lu, Ping Luo, Jiang Gu, Quanming Zou, Hao Zeng
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Mutant Libraries … MRSA COL (GI number: 57650036) genome, 20 overlapping peptides that spanned the entire length of SEB and consisted of 18 amino acid residues, three truncated peptides of SEB 85-102 , and six mutant peptides of SEB 85-102 were synthesized by GenScript Biotech Corp … Get A Quote

摘要

Staphylococci are the main cause of nosocomial infections globally. The exotoxin staphylococcal enterotoxin B (SEB) produced by methicillin-resistant is a major cause of pathology after a staphylococcal infection. We previously isolated an anti-SEB human monoclonal antibody designated as M0313. Here we further characterize this antibody in vitro and in vivo. Immunoblotting analysis and ELISA results indicated that M0313 accurately recognized and bound to SEB. Its binding affinity to native SEB was measured at the low nM level. M0313 effectively inhibited SEB from inducing mouse splenic lymphocyte and human peripheral blood mononuclear cell proliferation and cytokine release in cell culture. M0313 also neutrali... More

关键词

Methicillin-resistant Staphylococcus aureus, biological warfare, enterotoxin, immunotherapeutics, monoclonal antibody, superantigen