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Genomic and phylogenetic characteristics of a novel goose astrovirus in Anhui Province, Central-Eastern China

Gene. 2020-06-01; 
Yong Wang, Caixia Bai, Da Zhang, Kankan Yang, Zhaorong Yu, Shudong Jiang, Kai Ge, Yongdong Li
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DNA Sequencing … gel. After purification using the TIANgel Midi Purification Kit (Tiangen) in accordance with the manufacturer's instructions, the positive PCR products were sent to Genscript Biotech Corporation (Nanjing, China) for sequencing … Get A Quote

摘要

Goose astrovirus (GAstV) causes a novel disease characterized by urate deposition in the viscera and joints in goslings in many provinces of China, leading to huge economic losses in the goose industry. To better understand the genetic diversity of GAstV in the Anhui Province, Central-Eastern China, 48 kidney samples from goslings with gout were subjected to reverse-transcription polymerase chain reaction (RT-PCR) analysis for detecting GAstV, and phylogenetic analysis of whole genomes and ORFs was performed. Thirty-five samples were GAstV-positive, indicating that the virus is a frequent cause of gout. The whole genomes of 5 GAstV strains were successfully sequenced and named AHAU1-5. The sequenced genomes and... More

关键词

Anhui Province, Complete sequence, GAstV, Phylogenetic analysis