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CcpN: a moonlighting protein regulating catabolite repression of gluconeogenic genes in Bacillus subtilis also affects cell length and interacts with DivIVA

Can J Microbiol. 2020-08-01; 
Kusum Sharma, Taranum Sultana, Tanya Dahms, Jo-Anne R Dillon
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Monoclonal Antibody Services … 128 separated by SDS-PAGE, followed by sequential Western blot analysis using anti-GST first and 129 then anti-6×His monoclonal antibody (Genscript, USA) at a concentration of 0.3 µg/mL … 138 antibody was used for detecting GST-DivIVABs (Genscript, USA) at 0.3 µg/mL … Get A Quote

摘要

CcpN is a transcriptional repressor in Bacillus subtilis that binds to the promoter region of gapB and pckA, downregulating their expression in the presence of glucose. CcpN also represses sr1, which encodes a small non-coding regulatory RNA that suppresses the arginine biosynthesis gene cluster. CcpN has homologues in other Gram-positive bacteria including Enterococcus faecalis. We report the interaction of CcpN with DivIVA of B. subtilis as determined using Bacterial two-hybrid and GST pull-down assays. Insertional inactivation of CcpN leads to cell elongation and formation of straight chains of cells. These findings suggest that CcpN is a moonlighting protein involved in both gluconeogenesis and cell elongat... More

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