| Products/Services Used | Details | Operation |
|---|---|---|
| Monoclonal Antibody Services | … 128 separated by SDS-PAGE, followed by sequential Western blot analysis using anti-GST first and 129 then anti-6×His monoclonal antibody (Genscript, USA) at a concentration of 0.3 µg/mL … 138 antibody was used for detecting GST-DivIVABs (Genscript, USA) at 0.3 µg/mL … | Get A Quote |
CcpN is a transcriptional repressor in Bacillus subtilis that binds to the promoter region of gapB and pckA, downregulating their expression in the presence of glucose. CcpN also represses sr1, which encodes a small non-coding regulatory RNA that suppresses the arginine biosynthesis gene cluster. CcpN has homologues in other Gram-positive bacteria including Enterococcus faecalis. We report the interaction of CcpN with DivIVA of B. subtilis as determined using Bacterial two-hybrid and GST pull-down assays. Insertional inactivation of CcpN leads to cell elongation and formation of straight chains of cells. These findings suggest that CcpN is a moonlighting protein involved in both gluconeogenesis and cell elongat... More
