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Species-specific recruitment of transcription factors dictates toxin expression

Nucleic Acids Res. 2020-03-01; 
Julian Trouillon, Erwin Sentausa, Michel Ragno, Mylène Robert-Genthon, Stephen Lory, Ina Attrée, Sylvie Elsen
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GenParts™ DNA Fragments … enhancer analyses. The DNA fragments of actinobacterial genomes were synthesized by a company (GenScript Japan, Tokyo, Japan). The DNA fragments were inserted at the PstI site of the corresponding vectors. TALENs … Get A Quote

摘要

Tight and coordinate regulation of virulence determinants is essential for bacterial biology and involves dynamic shaping of transcriptional regulatory networks during evolution. The horizontally transferred two-partner secretion system ExlB-ExlA is instrumental in the virulence of different Pseudomonas species, ranging from soil- and plant-dwelling biocontrol agents to the major human pathogen Pseudomonas aeruginosa. Here, we identify a Cro/CI-like repressor, named ErfA, which together with Vfr, a CRP-like activator, controls exlBA expression in P. aeruginosa. The characterization of ErfA regulon across P. aeruginosa subfamilies revealed a second conserved target, the ergAB operon, with functions unrelated to ... More

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