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Characterization and functional analysis of chicken APOBEC4

Dev Comp Immunol. 2020-01-01; 
Mengyu Shi, Lei Tan, Yaodan Zhang, Chunchun Meng, Wei Wang, Yingjie Sun, Cuiping Song, Weiwei Liu, Ying Liao, Shengqing Yu, Tao Ren, Zhuang Ding, Xiufan Liu, Xusheng Qiu, Chan Ding
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GenParts™ DNA Fragments … Generation of targeting constructs To assemble the ovaCMV construct, a linear DNA fragment containing the CMV promoter, a polyA tail, the SV40 pro- moter, neomycin gene and a second polyA tail was synthesized (Genscript) and cloned into pUC57 using the EcoRV cloning … Get A Quote

摘要

The APOBEC proteins play significant roles in the innate and adaptive immune system, probably due to their deaminase activities. Because APOBEC1 (A1) and APOBEC3 (A3) are absent in the chicken genome, we were interested in determining whether chicken APOBEC4 (A4) possessed more complex functions than its mammalian homologs. In this study, chicken A4 (chA4) mRNA was identified and cloned for the first time. Based on bioinformatics analyses, the conserved zinc-coordinating motif (HXE … PC(X)C) was identified on the surface of chA4 and contained highly conserved His97, Glu99, Pro130, Cys131 and Cys138 active sites. The highest expression levels of constitutive chA4 were detected in primary lymphocytes and bursa ... More

关键词

APOBECs, Antiviral effect, Newcastle disease virus, chAPOBEC4