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Development of Dual Functional Nucleic Acid Delivery Nanosystem for DNA Induced Silencing of Bcl-2 Oncogene

Int J Nanomedicine. 2020-03-01; 
Somayeh Karimi, Mohamad Hassan Fouani, Ahmad Moshaii, Maryam Nikkhah, Saman Hosseinkhani, Reza Sheikhnejad
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Molecular Biology Tools GenScript Sequence Scramble10 was used to randomize the sequences and the National Center for Biotechnology Information (NCBI) Nucleotide Basic Local Alignment Search Tool (BLAST)11 was used to minimize sequence homology to the human mRNA transcript … Get A Quote

摘要

unassigned: Cancer treatment using functionalized vehicles in order to block involved genes has attracted a remarkable interest. In this study, we investigated the cellular uptake and cytotoxic effects of three sizes of anti Bcl-2 DNAi-conjugated gold nanoparticles by MCF-7 cells. unassigned: Three different sizes of gold nanoparticles were synthesized by citrate reduction method and after characterization, the nanoparticles were functionalized by Bcl-2 targeted DNAi. Cell internalization of the nanoparticles was analyzed by atomic absorption spectroscopy and light microscopy. The cytotoxic effects of the nanoparticles were investigated by MTT assay, flow cytometry and RT-PCR of the target gene. unassigned: Whi... More

关键词

Bcl-2, DNAi, apoptosis, breast cancer, gold nanoparticles