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Regulation of autophagy by systemic admission of microRNA-141 to target HMGB1 in l-arginine-induced acute pancreatitis in vivo

Pancreatology. 2016-03-01; 
Hongwei Zhu, Lihua Huang, Shaihong Zhu, Xia Li, Zhiqiang Li, Can Yu, Xiao Yu
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GenParts™ DNA Fragments … Plasmid construction. A 600-base-pair genomic fragment spanning the mmu-miR-141( GenePharma company, Shanghai, China) was inserted into pUC19 expression vector (Genscript Biotechnology Company, Nanjing, China) at its XhoI/BglII sites to express miR-141 … Get A Quote

摘要

objective: MicroRNAs are endogenous, non-coding RNAs of approximately 20-22 nucleotides that regulate gene expression by binding to the 3' untranslated region (UTR) of target mRNAs and can be applied in gene therapy. Acute pancreatitis is an inflammatory pancreatic disease that carries considerable morbidity and mortality. The purpose of this study was to explore the therapeutic potential of microRNA-141 (miR-141) for acute pancreatitis in vivo. methods: AP was induced by two hourly intra-peritoneal (i.p.) injections of l-arginine (200mg × 2/100 g.BW). Control mice received normal saline injections. In a separate group, normal saline, empty adenoviral vector and miR-141 adenoviral vector were given to the ... More

关键词

Acute pancreatitis, Autophagy, Gene therapy, microRNA-141