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Expression kinetics of ISG15, IRF3, IFNγ, IL10, IL2 and IL4 genes vis-a-vis virus shedding, tissue tropism and antibody dynamics in PPRV vaccinated, challenged, infected sheep and goats

Microb Pathog. 2018-02-01; 
Sajad Ahmad Wani, Amit Ranjan Sahu, Shikha Saxena, Kaushal Kishor Rajak, M Saminathan, Aditya Prasad Sahoo, Sonam Kanchan, Aruna Pandey, Bina Mishra, D Muthuchelvan, Ashok Kumar Tiwari, Bishnu Prasad Mishra, Raj Kumar Singh, Ravi Kumar Gandham
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GenParts™ DNA Fragments … Quantification by real-time RT-PCR was performed using primers specific to PPRV that were designed using Genscript software (USA). The expected 130 bp fragment, covering part of Peste des Petits Ruminants in Sheep and Goats PLOS ONE | www.plosone.org … Get A Quote

摘要

Here, we studied the in vivo expression of Th1 (IL2 and IFN gamma) and Th2 (IL4 and IL10) - cytokines and antiviral molecules - IRF3 and ISG15 in peripheral blood mononuclear cells in relation to antigen and antibody dynamics under Peste des petits ruminants virus (PPRV) vaccination, infection and challenge in both sheep and goats. Vaccinated goats were seropositive by 9 days post vaccination (dpv) while in sheep idiosyncratic response was observed between 9 and 14 dpv for different animals. Expression of PPRV N gene was not detected in PBMCs of vaccinated and vaccinated challenged groups of both species, but was detected in unvaccinated infected PBMCs at 9 and 14 days post infection. The higher viral load at 9... More

关键词

Gene expression, PPR, Tropism, qRT-PCR