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GenParts™ DNA Fragments> | … Fragments of the 16S rRNA genes were amplified by PCR using the primers 27F and 1492R as described previously (Lane, 1991). PCR products were purified and sequenced by GenScript. We thus obtained nearly full-length 16S rRNA gene sequences of all isolated bacteria … | Get A Quote |
A bacterial strain, designated 17SD1-15, was isolated from soil. Cells of this strain were Gram-stain-negative and aerobic rods. The major fatty acids of strain 17SD1-15 were iso-C15 : 0 and summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I). The polar lipids were phosphatidylethanolamine, one phospholipid and five unidentified lipids. The G+C content of the genomic DNA of strain 17SD1-15 was 49.0 mol%. The 16S rRNA gene sequence analysis showed that strain 17SD1-15 was phylogenetically related to Pontibacter saemangeumensis GCM0142, Pontibacter korlensis X14-1, Pontibacter yuliensis H9X, Pontibacter diazotrophicus H4X and Pontibacter humi SWU8 (98.3, 96.4, 96.4, 96.4 and 96.0 % sequenc... More