至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A Heterologous Viral Protein Scaffold for Chimeric Antigen Design: An Example PCV2 Virus Vaccine Candidate

Viruses. 2020-03-01; 
Emilio Lamazares, Fernando Gutiérrez, Angela Hidalgo, Nicolas A Gutiérrez, Felipe I Espinoza, Oliberto Sánchez, Marcelo Cortez-San Martín, Carolina Mascayano, Javier González, José Saavedra, Claudia Altamirano, Manuel Mansur, Álvaro Ruiz, Jorge R Toledo
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PCR Cloning and Subcloning … 2. Materials and Methods 2.1. Plasmid, Gene Cloning, and Strain The synthetic gene of chimera 1 (Qm1) GenScript (Nanjing, China) was cloned into XhoI-NdeI sites of the vector pET-22b (+). E. coli strain SHuffle ® T7 Express (New England Biolabs, Ipswich, MA … Get A Quote

摘要

Recombinant vaccines have low-cost manufacturing, regulatory requirements, and reduced side effects compared to attenuated or inactivated vaccines. In the porcine industry, post-weaning multisystemic disease syndrome generates economic losses, characterized by progressive weight loss and weakness in piglets, and it is caused by porcine circovirus type 2 (PCV2). We designed a chimeric antigen (Qm1) to assemble the main exposed epitopes of the Cap-PCV2 protein on the capsid protein of the tobacco necrosis virus (TNV). This design was based on the Cap-N-terminal of an isolated PCV2 virus obtained in Chile. The virus was characterized, and the sequence was clustered within the PCV2 genotype b clade. This chimeric p... More

关键词

PCV2 virus, biotechnology strategies, recombinant antigens production, vaccines