至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Broad-specificity GH131 β-glucanases are a hallmark of fungi and oomycetes that colonize plants

Environ Microbiol. 2019-04-01; 
George E Anasontzis, Marc-Henri Lebrun, Mireille Haon, Charlotte Champion, Annegret Kohler, Nicolas Lenfant, Francis Martin, Richard J O'Connell, Robert Riley, Igor V Grigoriev, Bernard Henrissat, Jean-Guy Berrin, Marie-Noëlle Rosso
Products/Services Used Details Operation
PCR Cloning and Subcloning … 2016). Enzyme cloning, production and purification The coding sequences of the selected GH131s were optimised for Pichia pastoris, synthesized and cloned into the pPICZαA plasmid by Genscript (NJ, USA), so that open reading frames were in … Get A Quote

摘要

Plant-tissue-colonizing fungi fine-tune the deconstruction of plant-cell walls (PCW) using different sets of enzymes according to their lifestyle. However, some of these enzymes are conserved among fungi with dissimilar lifestyles. We identified genes from Glycoside Hydrolase family GH131 as commonly expressed during plant-tissue colonization by saprobic, pathogenic and symbiotic fungi. By searching all the publicly available genomes, we found that GH131-coding genes were widely distributed in the Dikarya subkingdom, except in Taphrinomycotina and Saccharomycotina, and in phytopathogenic Oomycetes, but neither other eukaryotes nor prokaryotes. The presence of GH131 in a species was correlated with its associati... More

关键词