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Hypoxia preconditioning promotes bone marrow mesenchymal stem cells survival by inducing HIF-1α in injured neuronal cells derived exosomes culture system

Cell Death Dis. 2019-02-01; 
Zucheng Luo, Fangfang Wu, Enxing Xue, Linlin Huang, Ping Yan, Xiaoyun Pan, Yulong Zhou
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DNA Sequencing … The correct sequence was further verified by sequencing. The blank vector was also prepared as a negative control for transfection (BC). The specific small interfering RNA (siRNA) for Hif1a and the negative siRNA control (siNC) were synthesized by Genscript (Nanjing, China) … Get A Quote

摘要

Bone marrow derived stem cells (BMSCs) transplantation are viewed as a promising therapeutic candidate for spinal cord injury (SCI). However, the inflammatory microenvironment in the spinal cord following SCI limits the survival and efficacy of transplanted BMSCs. In this study, we investigate whether injured neuronal cells derived exosomes would influence the survival of transplanted BMSCs after SCI. In order to mimic the microenvironment in SCI that the neuronal cells or transplanted BMSCs suffer in vivo, PC12 cells conditioned medium and PC12 cell's exosomes collected from HO-treated PC12 cell's culture medium were cultured with BMSCs under oxidative stress in vitro. PC12 cells conditioned medium and PC12 ce... More

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