至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Iterative cycle of widely targeted metabolic profiling for the improvement of 1-butanol titer and productivity in

Biotechnol Biofuels. 2018-07-01; 
Artnice Mega Fathima, Derrick Chuang, Walter Alvarez Laviña, James Liao, Sastia Prama Putri, Eiichiro Fukusaki
Products/Services Used Details Operation
Monoclonal Antibody Services … western blot analysis. Protein bands were probed by rabbit anti-GGCX polyclonal antibody (Proteintech Group Inc., Chicago, IL) and mouse anti-tubulin monoclonal anti- body (GenScript Inc., Piscataway, NJ). Cell-based GGCX … Get A Quote

摘要

unassigned: Metabolomics is the comprehensive study of metabolites that can demonstrate the downstream effects of gene and protein regulation, arguably representing the closest correlation with phenotypic features. Hence, metabolomics-driven approach offers an effective way to facilitate strain improvement. Previously, targeted metabolomics on the 1-butanol-producing cyanobacterial strain BUOHSE has revealed the reduction step from butanoyl-CoA to butanal, catalyzed by CoA-acylating propionaldehyde dehydrogenase (PduP), as a rate-limiting step in the CoA-dependent pathway. Moreover, an increase in acetyl-CoA synthesis rate was also observed in this strain, by which the increased rate of release of CoA from but... More

关键词

Butanol, Cyanobacteria, Rate-limiting step, Strain improvement, Widely targeted metabolomics