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DNA Sequencing> | … sequenced using the 96gIII sequencing primer. 2.7. Phage Design and Sequence Analysis. Peptides Pep0 and PepN were synthesized (purity >95%) by GenScript China Inc (Nanjing, Jiangsu, China). The amino acid sequence of … | Get A Quote |
Duck hepatitis A virus (DHAV), the major pathogen of duck virus hepatitis (DVH), causes severe diseases that threaten the duck industry worldwide. The VP1 protein, a major structural protein of DHAV, is able to induce neutralizing antibody in ducks. The purpose of this study was to identify the antigenic mimotope of DHAV by phage display technology. A monoclonal antibody (mAb) 4E6 against DHAV-1 and DHAV-3 was prepared, and a phage library prepared with the PhD-12 Phage Display Peptide Library Kit was screened with the mAb. A novel peptide, GLTWKLPPSM was identified with high affinity to the mAb and could specifically block mAb 4E6 from binding DHAV-1 and DHAV-3. Animal tests confirmed that the immunization of ... More