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A Novel Peptide Isolated from a Phage Display Peptide Library Modeling Antigenic Epitope of DHAV-1 and DHAV-3

Vaccines (Basel). 2020-03-01; 
Ruihua Zhang, Yupeng Yang, Jingjing Lan, Shaoli Lin, Zhijing Xie, Xiansheng Zhang, Shijin Jiang
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DNA Sequencing … sequenced using the 96gIII sequencing primer. 2.7. Phage Design and Sequence Analysis. Peptides Pep0 and PepN were synthesized (purity >95%) by GenScript China Inc (Nanjing, Jiangsu, China). The amino acid sequence of … Get A Quote

摘要

Duck hepatitis A virus (DHAV), the major pathogen of duck virus hepatitis (DVH), causes severe diseases that threaten the duck industry worldwide. The VP1 protein, a major structural protein of DHAV, is able to induce neutralizing antibody in ducks. The purpose of this study was to identify the antigenic mimotope of DHAV by phage display technology. A monoclonal antibody (mAb) 4E6 against DHAV-1 and DHAV-3 was prepared, and a phage library prepared with the PhD-12 Phage Display Peptide Library Kit was screened with the mAb. A novel peptide, GLTWKLPPSM was identified with high affinity to the mAb and could specifically block mAb 4E6 from binding DHAV-1 and DHAV-3. Animal tests confirmed that the immunization of ... More

关键词

duck hepatitis A virus, immunization, mimitope, monoclonal antibody, phage display